Phytochemical Screening and Quality of Crescentia cujete L. Leaf Extract

Authors

  • ID Julia Putri Ayunda Jurusan Analisis Farmasi dan Makanan (Anafarma), Poltekkes Kemenkes Surakarta Indonesia Author
  • ID Makhabbah Jamilatun Jurusan Analisis Farmasi dan Makanan (Anafarma), Poltekkes Kemenkes Surakarta Indonesia Author
  • ID Pradea Indah Lukito Jurusan Analisis Farmasi dan Makanan (Anafarma), Poltekkes Kemenkes Surakarta Indonesia Author

DOI:

https://doi.org/10.66914/9c4qsa10

Keywords:

phytochemical, screening, Crescentia cujete L., leaf, extract

Abstract

Crescentia cujete L., known as maja, has traditionally been used as a medicinal plant in several countries. The fruit and other parts of this plant contain bioactive compounds with antioxidant, antibacterial, antidiabetic, anti-inflammatory, and cytotoxic activities related to their secondary metabolites. The purpose of this study was to determine the quality of Crescentia cujete L. leaf extract obtained from Gajahan Hamlet, Pasuruan. This study used an experimental method, which included organoleptic tests, water- and ethanol-soluble extract levels, water content, ash content, and phytochemical screening. The results showed that the yield of Crescentia cujete L. leaf extract was 13.2939%. The quality parameters of Crescentia cujete L. leaf extract included organoleptic tests such as thick extract, odor according to the plant of origin, brown color, water-soluble extract content of 11.54%, ethanol-soluble extract content of 12.49%, water content of 8.44%, and ash content of 4.12%. These results indicate that Crescentia cujete L. leaf extract meets the requirements according to the Indonesian Herbal Pharmacopoeia. The results of phytochemical screening of Crescentia cujete L. leaf extract indicate that Crescentia cujete L. leaf extract contains flavonoids, alkaloids, saponins, tannins, and steroids

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References

Balogun, Fatai Oladunni, & Sabiu, Saheed. (2021). A Review of the Phytochemistry, Ethnobotany, Toxicology, and Pharmacological Potentials of Crescentia cujete L. (Bignoniaceae). Evidence-Based Complementary and Alternative Medicine, Vol. 2021. https://doi.org/10.1155/2021/6683708

Edo, Great Iruoghene, Obasohan, Promise, Makia, Raghda S., Abiola O, Tubi, Umelo, Ebuka Chukwuma, Jikah, Agatha Ngukuran, Yousif, Emad, Isoje, Endurance Fegor, Igbuku, Ufuoma Augustina, Opiti, Rapheal Ajiri, Essaghah, Arthur Efeoghene Athan, Ahmed, Dina S., & Umar, Huzaifa. (2024). The use of quality control parameters in the evaluation of herbal drugs. A review. Discover Medicine, 1(1). https://doi.org/10.1007/s44337-024-00177-6

Gaspersz, Nelson, Kapelle, Imanuel B. D., Hattu, Nikmans, Sangadji, Insun, & Arpipi, Henderika S. S. (2024). Chemicals Identification in The Ethyl Acetate Fraction and The Antioxidant Activity from Calabash Seed (Cresentia cujete) Extract. HAYATI Journal of Biosciences, 31(6). https://doi.org/10.4308/hjb.31.6.1144-1153

Gonzales, Alecsanndra L., Sevilla, Ureah Thea A., & Tsai, Po Wei. (2023). Pharmacological Activities of Bioactive Compounds from Crescentia cujete L. Plant – A Review. Biointerface Research in Applied Chemistry, Vol. 13. https://doi.org/10.33263/BRIAC132.197

Jamilatun, Makhabbah. (2023). Isolation and Phytochemical Testing of Endophytic Fungi from Imperata cylindrica ( L .) Beauv plants . International Journal of Basic and Applied Science, 12(2), 62–71. https://doi.org/https://doi.org/10.35335/ijobas.v12i2.255

Jamilatun, Makhabbah, & Lukito, Pradea Indah. (2022). Pendidikan Kesehatan tentang Pangan Fungsional Olahan Buah Naga Merah kepada Ibu-Ibu PKK di Desa Tegalyoso Klaten Selatan. Joong-Ki : Jurnal Pengabdian Masyarakat, 1(3), 343–348. https://doi.org/https://doi.org/10.56799/joongki.v1i3.724

Jamilatun, Makhabbah, & Lukito, Pradea Indah. (2025). Formulation of Edible Film Mucoadhesive Cinnamon Extract (Cinnamomum burmanni). Sasambo Journal of Pharmacy, 6(2), 86–93. https://doi.org/10.29303/sjp.v6i2.579

Jamilatun, Makhabbah, Lukito, Pradea Indah, & Kumalasari, Intan. (2026). Quality Analysis of Leaf and Stem Extracts of the Mikania micrantha. 4(01), 64–75.

Jamilatun, Makhabbah, Lukito, Pradea Indah, & Rayhanissa, Redita Azzahra Dee. (2025). Antioxidant Activity of Peel-Off Gel Mask of Aloe Vera and Strawberry. Quantum Journal of Medical and Health Sciences (QJMHS), 4(2), 70–77. https://doi.org/https://doi.org/10.55197/qjmhs.v4i2.154

Jamilatun, Makhabbah, Lukito, Pradea Indah, & Zahra, Melda Aulia. (2025). Antioxidant Activity of Bay (Syzygium polyanthum [Wight] Walp) Leaf Extract Toner. Journal of Health Science and Medical Therapy, 3(03), 255–264. https://doi.org/10.59653/jhsmt.v3i03.1853

Jamilatun, Makhabbah, Purnamasari, Tiara, & Tolkhah, Ronal. (2024). Jelly Candy Production With Variations of Broccoli (Brassica oleracea var. italica) and Red Ginger (Zingiber officinale var. rubrum Theilade) 1. Jurnal Jamu Kusuma, 4(1), 14–18. https://doi.org/https://doi.org/10.37341/jurnaljamukusuma.v4i1.73

Jamilatun, Makhabbah, Utami, Evi Dewi, & Tolkhah, Ronal. (2025). Antifungal Activity of Hair Dyes Gel from Henna Leaves and Clove Flower. Indonesian Journal of Pharmaceutical (e-Journal), 5(1), 1–11. https://doi.org/10.37311/ijpe.v5i1.27246

Kementerian Kesehatan RI. (2023). Farmakope Herbal Indonesia Edisi II Tahun 2017. Pills and the Public Purse.

Khairi, Nur, Hapiwaty, Saldi, Yusuf, Sepriani, & Indrisari, Maulita. (2023). Pengukuran Parameter Spesifik dan Non Spesifik Ekstrak Etanol Beras Hitam (Oryza sativa L. indica) Asal Toraja. Jurnal Katalisator, 8(2).

Khan, Rahim, Anwar, Farooq, Ghazali, Farinazleen Mohamad, & Mahyudin, Nor Ainy. (2024). Valorization of waste: Innovative techniques for extracting bioactive compounds from fruit and vegetable peels - A comprehensive review. Innovative Food Science and Emerging Technologies, Vol. 97. https://doi.org/10.1016/j.ifset.2024.103828

Lukman, Muhammad. (2020). Analisa Keamanan Produk Pangan Metode Hazard Analysis Critical Control Point Langkah 1-6. Seminar Nasional Teknologi Dan Rekayasa (SENTRA), 4(1).

Manongko, Paricia Syaron, Sangi, Meiske Sientje, & Momuat, Lidya Irma. (2020). Uji Senyawa Fitokimia dan Aktivitas Antioksidan Tanaman Patah Tulang (Euphorbia tirucalli L.). Jurnal MIPA, 9(2). https://doi.org/10.35799/jmuo.9.2.2020.28725

Materia Medica. (2025). Determinasi. Malang, Jawa Timur: UPT Materia Medika Batu Malang.

Messinese, Edmondo, Pitirollo, Olimpia, Grimaldi, Maria, Milanese, Daniel, Sciancalepore, Corrado, & Cavazza, Antonella. (2024). By-Products as Sustainable Source of Bioactive Compounds for Potential Application in the Field of Food and New Materials for Packaging Development. Food and Bioprocess Technology, Vol. 17. https://doi.org/10.1007/s11947-023-03158-2

Morales, Diego. (2025). Insights on the Health Benefits and Functional Potential of Food Bioactive Compounds. Foods, Vol. 14. https://doi.org/10.3390/foods14111984

Nabila Nur Latifa, Lanny Mulqie, & Siti Hazar. (2022). Penetapan Kadar Sari Larut Air dan Kadar Sari Larut Etanol Simplisia Buah Tin (Ficus carica L.). Bandung Conference Series: Pharmacy, 2(2). https://doi.org/10.29313/bcsp.v2i2.4575

Nurhasanah, Harlina, & Adhitiyawarman. (2014). Uji Bioaktivitas Ekstrak Daun Maja (Crescentia cujete Linn) Sebagai Anti Rayap. Jurnal Kimia Khatulistiwa, 3(3).

Pratiwi, M. N. (2019). Aktivitas Antibakteri Fraksi Buah Jambu Wer (Prunus persica (L.) Batsch) Terhadap Pertumbuhan Bakteri Staphylococcus aureus. Skripsi.

Rahmatia, Lulu, Nasrudin, & Nurlansi. (2022). Fitokimia dan Aktivitas Antiradikal DPPH Seduhan Daun Salam. Jurnal Ilmu Kimia Dan Pendidikan Kimia, 11.

Rahmawati, Aulya Fitria. (2022). Uji Aktivitas Antibakteri Fraksi Daun Majapahit (Crescentia cujete L.) terhadap Bakteri Staphylococcus aureus secara In Vitro. Braz Dent J., 33(1).

Ren, Xinghai, Zhang, Guangzhong, Jin, Mengjiao, Wan, Fanghao, Day, Michael D., Qian, Wanqiang, & Liu, Bo. (2023). Metabolomics and Transcriptomics Reveal the Response Mechanisms of Mikania micrantha to Puccinia spegazzinii Infection. Microorganisms, 11(3), 1–15. https://doi.org/10.3390/microorganisms11030678

Royani, Sri, Febri Fatwami, Esti, Islamiyati, Dian, & Stasiana Yunarti, Kresensia. (2024). Uji Kandungan Fitokimia pada Daun Salam (Syzygium polyanthum) di Kabupaten Banyumas. In Jurnal Bina Cipta Husada.

Supriningrum, Risa, Sundu, Reksi, Sentat, Triswanto, Niah, Rakhmadhan, & Kumalasari, Eka. (2021). Karakterisasi Simplisia Dan Ekstrak Kulit Batang Sekilang (Embelia borneensis Scheff.). Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi Dan Kesehatan, 6(2). https://doi.org/10.36387/jiis.v6i2.677

Ulandari, Atri Sri, & Sani, Sulwiyatul Kamariyah. (2023). Identifikasi Senyawa Metabolit Sekunder Ekstrak Metanol Daun dan Kulit Batang Banten ( Lannea coromandelica ) Menggunakan GC-MS Sebagai Tanaman Obat. Jurnal Ilmu Kefarmasian, 4(1).

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Published

2026-08-08

Issue

Vol. 2 No. 8 (2026): Sinergi: Jurnal Ilmiah Multidisiplin

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Articles

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Copyright (c) 2026 Julia Putri Ayunda, Makhabbah Jamilatun, Pradea Indah Lukito (Author)

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